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Catalog No: OKEH01527
Size:96T
Price: $600.00
SKU
OKEH01527
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology WISP2 ELISA Kit (Mouse) (OKEH01527) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WISP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WISP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WISP2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
WISP2 Microplate96 Wells (12 x 8 Well strips)
WISP2 Lyophilized Standard2
100X Biotinylated WISP2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.095 ng/mL
SpecificityNatural and recombinant Mouse WNT1-inducible-signaling pathway protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCcn5
Gene Full Namecellular communication network factor 5
Alias SymbolsCCN;CCN family member 5;connective tissue growth factor-like protein;Crg;Crgr4;Ctgfl;CTGF-L;rC;Rcop1;Wi;Wisp2;WISP-2;WNT1 inducible signaling pathway protein 2.
NCBI Gene Id22403
Protein NameQ9Z0G4
Description of TargetWNT1-inducible-signaling pathway protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Z0G4
Protein Accession #NP_058569.2
Nucleotide Accession #NM_016873.2
Protein Size (# AA)251
Molecular Weight27 kDa
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