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Catalog No: OKEH06456
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology WISP2 ELISA Kit (Human) (OKEH06456) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WISP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WISP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WISP2 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 104%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 6.1% (n = 20)
Mean Inter-Assay Precision - <= 8.4% (n = 20)
ELISA Kit Component
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.094 ng/mL
SpecificityNatural and recombinant Human WNT1-inducible-signaling pathway protein 2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCCN5
Gene Full Namecellular communication network factor 5
Alias SymbolsCCN family member 5;connective tissue growth factor-like protein;connective tissue growth factor-related protein 58;CT58;CTGF-L;WISP2;WNT1 inducible signaling pathway protein 2.
NCBI Gene Id8839
Protein NameO76076
Description of TargetWNT1-inducible-signaling pathway protein 2
Uniprot ID
Protein Accession #NP_002436
Nucleotide Accession #NM_002445
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