WISP1/CCN4 ELISA Kit (Human) (OKBB00538)

Catalog No: OKBB00538

Size:96 Wells

Human WISP1/CCN4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human WISP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for WISP1 has been precoated onto 96-well plates. Standards(NS0, T23-N367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WISP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human WISP1 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	236	1087	1826	352	1240	1910
  SD	7.55	46.7	89.5	16.2	64.5	128
  CV%	3.2	4.3	4.9	4.6	5.2	6.7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human CCN4 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human CCN4 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human CCN4 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human WISP1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: T23-N367
• Description: For quantitative detection of human WISP1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCN4
• Gene Full Name: cellular communication network factor 4
• Alias Symbols: CCN family member 4, CCN4, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 inducible signaling pathway protein 1, Wnt-1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, WNT1 inducible-signaling pathway protein 1
• NCBI Gene Id: 8840
• Protein Name: CCN family member 4
• Description of Target: WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
• Uniprot ID: O95388
• Protein Accession #: NP_001191798.1
• Nucleotide Accession #: NM_001204869.1