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Catalog No: OKEH03297
Size:96T
Price: $525.00
SKU
OKEH03297
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology WFIKKN1 ELISA Kit (Human) (OKEH03297) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WFIKKN1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for WFIKKN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WFIKKN1 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.9% (n = 20)
Mean Inter-assay CV%: <= 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-WFIKKN1 Microplate96 Wells (12 x 8 Well strips)
WFIKKN1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated WFIKKN1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Human WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolWFIKKN1
Gene Full NameWAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1
Alias SymbolsC16orf12;GASP-2;growth and differentiation factor-associated serum protein 2;hGASP-2;RJD2;WAP four-disulfide core domain 20A;WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing protein;WAP, FS, Ig, KU, and NTR-containing protein;WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1;WFDC20A;WFIKKN.
NCBI Gene Id117166
Protein NameQ96NZ8
Description of TargetWAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q96NZ8
Protein Accession #NP_444514.1
Nucleotide Accession #NM_053284.2
Protein Size (# AA)548
Molecular Weight59 kDa
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