WFDC5 ELISA Kit (Human) (OKCD01822)

Catalog No: OKCD01822 (Formerly GWB-KBBXD4)

Size:96 Wells

Human WFDC5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-103%	80-101%	96-103%	99-105%
  EDTA Plasma (n=5)	84-102%	90-105%	84-97%	97-104%
  Heparin Plasma (n=5)	80-103%	81-104%	80-90%	80-95%

• ELISA Kit Principle: Aviva Systems Biology WFDC5 ELISA Kit (Human) (OKCD01822) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WFDC5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WFDC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample WFDC5 captured in the well.
• ELISA Kit Range: 78.1 - 5,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	96-104	101
  EDTA Plasma (n=5)	92-99	95
  Heparin Plasma (n=5)	94-101	98

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level WFDC5 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level WFDC5 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-WFDC5 Microplate: 96 Wells (12 x 8 Well Strips)
  • WFDC5 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated WFDC5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 35 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of WAP Four Disulfide Core Domain Protein 5 (WFDC5). No significant cross-reactivity or interference between WAP Four Disulfide Core Domain Protein 5 (WFDC5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: WFDC5
• Gene Full Name: WAP four-disulfide core domain 5
• Alias Symbols: PRG5, WAP1, dJ211D12.5
• NCBI Gene Id: 149708
• Protein Name: WAP four-disulfide core domain protein 5
• Description of Target: Putative acid-stable proteinase inhibitor.
• Uniprot ID: Q8TCV5
• Protein Accession #: NP_663627.1
• Nucleotide Accession #: NM_145652.3
• Protein Size (# AA): 224