WFDC2 Recombinant Antibody [17D2] (OACA12856)

Catalog No: OACA12856

Size:100UL

Western Blot
Positive WB detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate
All lanes: HE4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa

Overlay Peak curve showing MCF-7 cells stained with OACA12856 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Flow cytometry,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from Human WFDC2
• Purification: Affinity Purified

Target Info

• Gene Symbol: WFDC2
• Gene Full Name: WAP four-disulfide core domain 2
• Alias Symbols: dJ461P17.6;EDDM4;epididymal protein 4;epididymal secretory protein E4;epididymis secretory sperm binding protein;epididymis-specific, whey-acidic protein type, four-disulfide core;HE4;human epididymis protein 4;major epididymis-specific protein E4;putative protease inhibitor WAP5;WAP domain containing protein HE4-V4;WAP four-disulfide core domain protein 2;WAP5.
• NCBI Gene Id: 10406
• Protein Name: WAP four-disulfide core domain protein 2
• Description of Target: Broad range protease inhibitor.
• Uniprot ID: Q14508