Catalog No: OKCD06172
Size:96WELLS
Price: $600.00
SKU
OKCD06172
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for WFDC2 ELISA Kit (Human) (OKCD06172) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WAP Four Disulfide Core Domain Protein 2 (WFDC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WAP Four Disulfide Core Domain Protein 2 (WFDC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WAP Four Disulfide Core Domain Protein 2 (WFDC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WAP Four Disulfide Core Domain Protein 2 (WFDC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WAP Four Disulfide Core Domain Protein 2 (WFDC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WAP Four Disulfide Core Domain Protein 2 (WFDC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, saliva and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.126ng/mL |
Gene Symbol | WFDC2 |
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Gene Full Name | WAP four-disulfide core domain 2 |
Alias Symbols | dJ461P17.6;EDDM4;epididymal protein 4;epididymal secretory protein E4;epididymis secretory sperm binding protein;epididymis-specific, whey-acidic protein type, four-disulfide core;HE4;human epididymis protein 4;major epididymis-specific protein E4;putative protease inhibitor WAP5;WAP domain containing protein HE4-V4;WAP four-disulfide core domain protein 2;WAP5. |
NCBI Gene Id | 10406 |
Protein Name | WAP four-disulfide core domain protein 2 |
Description of Target | Broad range protease inhibitor. |
Uniprot ID | Q14508 |
Protein Accession # | NP_006094.3 |
Nucleotide Accession # | NM_006103.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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