WDR77 Antibody (OAAB22256)

Catalog No: OAAB22256

Size:100ul

Western blot detection of WDR77 in C6, 3T3 and K562 cell lysates using WDR77 Antibody (OAAB22256) (1:1,000 diluted). Predicted band size: 42KDa. Observed band size: 42KDa.

Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0. 1% Triton X-100, next using WDR77 mouse mAb (dilution 1:100).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC
• Additional Information: Function: Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
  ICC: 1:100
• Reference: Friesen W.J.,et al.J. Biol. Chem. 277:8243-8247(2002).
  Hosohata K.,et al.Mol. Cell. Biol. 23:7019-7029(2003).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Yamada S.,et al.Oncogene 23:5901-5911(2004).
  Gregory S.G.,et al.Nature 441:315-321(2006).
  

Target Info

• Gene Symbol: WDR77
• Gene Full Name: WD repeat domain 77
• Alias Symbols: p44, MEP50, MEP-50, HKMT1069, Nbla10071, p44/Mep50
• NCBI Gene Id: 79084
• Description of Target: The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q9BQA1
• Protein Accession #: NP_077007.1
• Nucleotide Accession #: NM_024102.3
• Molecular Weight: 37 kDa