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Catalog No: OCOA07939
Size:20UG
Price: $179.00
SKU
OCOA07939
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WDR61 Protein Lysate (OCOA07939)

Datasheets/ManualsPrintable datasheet for WDR61 Protein Lysate (OCOA07939)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionWDR61 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolWDR61
Gene Full NameWD repeat domain 61
Alias Symbolsmeiotic recombination REC14 protein homolog;REC14;recombination protein REC14;SKI8;SKI8 homolog;WD repeat-containing protein 61.
NCBI Gene Id80349
Protein NameNM_025234, NP_079510, Q9GZS3
Description of TargetHomo sapiens WD repeat domain 61 (WDR61), transcript variant 2, mRNA, WD repeat-containing protein 61, WD repeat-containing protein 61 isoform a [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q9GZS3
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_079510
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_025234
Molecular Weight33.6 kDa
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