WDR61 Antibody (OAGA05543)

Catalog No: OAGA05543

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
diluted at 1:500

Sample (50 ug of whole cell lysate)
A: mouse stomach
12% SDS PAGE
diluted at 1:1000

WDR61 antibody detects WDR61 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: WDR61 protein stained by WDR61 antibody diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human WDR61. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.6 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: WDR61
• Gene Full Name: WD repeat domain 61
• Alias Symbols: SKI8, REC14
• NCBI Gene Id: 80349
• Protein Name: WD repeat-containing protein 61
• Description of Target: WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM, Mar 2008]
• Uniprot ID: Q9GZS3
• Protein Accession #: NP_001290176.1
• Nucleotide Accession #: NM_001303247.1