WDR61 Antibody - N-terminal (OAPB01959)

Catalog No: OAPB01959

Size:100UG

Western blot analysis of WDR61 in SK-N-SH cell lysate with WDR61 antibody at 1 ug/ml.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Human|Mouse|Rat
• Product Format: Liquid. WDR61 antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: WDR61 antibody was raised against a 14 amino acid peptide near the amino terminus of human WDR61. The immunogen is located within the first 50 amino acids of WDR61.
• Purification: Affinity Purified
• Concentration: 1 mg/ml

Target Info

• Gene Symbol: WDR61
• Gene Full Name: WD repeat domain 61
• Alias Symbols: meiotic recombination REC14 protein homolog;REC14;recombination protein REC14;SKI8;SKI8 homolog;WD repeat-containing protein 61.
• NCBI Gene Id: 80349
• Protein Name: WD repeat-containing protein 61|WD repeat-containing protein 61 isoform a [Homo sapiens]
• Description of Target: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
• Uniprot ID: Q9GZS3
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_079510
• Nucleotide Accession #: NM_001303247.1
• Molecular Weight: Predicted: 34 kDa Observed: 30 kDa