WBSCR22 Antibody (OAGA00436)

Catalog No: OAGA00436

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HeLa
B: HCT116
12% SDS PAGE
OAGA00436 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (OAGA00436) antibody at 1:100 dilution.

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 ?g of WBSCR22 antibody (OAGA00436) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (OAGA00436) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, IP, WB
• Additional Information: Background: This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]
  Positive Controls: HeLa, HCT116
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 263 of WBSCR22 (Uniprot ID#O43709)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.42 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, IP: 1:500-1:1000.ICC/IF:Use at an dependent assay. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: WBSCR22
• Gene Full Name: Uncharacterized methyltransferase WBSCR22
• Alias Symbols: WBMT, MERM1, PP3381, HUSSY-3, WBSCR22, HASJ4442
• NCBI Gene Id: 114049
• Protein Name: Uncharacterized methyltransferase WBSCR22
• Uniprot ID: O43709
• Protein Accession #: NP_001189489.1
• Molecular Weight: 32 kDa