WARS ELISA Kit (Mouse) (OKCD02987)

Catalog No: OKCD02987

Size:96WELLS

Mouse WARS ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  	97-105%	80-99%	80-99%	80-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophanyl tRNA Synthetase (WARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophanyl tRNA Synthetase (WARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophanyl tRNA Synthetase (WARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tryptophanyl tRNA Synthetase (WARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  	80-88	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptophanyl tRNA Synthetase (WARS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptophanyl tRNA Synthetase (WARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-WARS Microplate: 96 Wells (12 x 8 Well strips)
  • WARS Lyophilized Standard: 2
  • 100X Biotinylated WARS Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tryptophanyl tRNA Synthetase (WARS). No significant cross-reactivity or interference between Tryptophanyl tRNA Synthetase (WARS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Wars
• Gene Full Name: tryptophanyl-tRNA synthetase
• Alias Symbols: TrpRS;tryptophan--tRNA ligase, cytoplasmic;Tryptophanyl-tRNA synthetase;tryptophanyl-tRNA synthetase, cytoplasmic;Wars1;WRS.
• NCBI Gene Id: 22375
• Protein Name: Tryptophan--tRNA ligase, cytoplasmic
• Description of Target: T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).
• Uniprot ID: P32921
• Protein Accession #: NP_001157786.1
• Nucleotide Accession #: NM_001164314.1
• Protein Size (# AA): 481