Catalog No: OASA03379 (Formerly GWB-147892)
Size:200UG
Price: $459.00
SKU
OASA03379
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsPrintable datasheet for VWF Antibody (OASA03379)
Product Info
Predicted Species ReactivityHuman
Product FormatLiquid. Phosphate buffered saline
ClonalityMonoclonal
CloneRFF-VIII R/1
IsotypeIgG1
HostMouse
ApplicationEnzyme-linked immunosorbent assay|Immunohistochemistry-Frozen|Purification|Radioimmunoassay
Additional InformationHistology Positive Control Tissue: Human tonsil, thymus, liver, spleen or kidney.
::Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
::Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage-20°C
ImmunogenHuman Factor VIII complex partially purified from Factor VIII concentrate.
PurificationProtein G Purified
Predicted Homology Based on Immunogen SequenceHuman
Concentration1 mg/ml
SpecificityVON WILLEBRAND FACTOR
Application InfoImmunohistology - Paraffin(1):    1/50 - 1/200
(1) Special conditions apply - see [*]
Application DataApplication #1: Staining of paraffin embedded human liver sections with Mouse anti human Von Willebrand Factor
Protocol InformationSpecies: Human
Experiment Name: Antibodies and Control Experiments
Experiment Background: To test for depletion of endothelial cells, the sections were studied with antibodies to von Willebrand factor, and no differences were found between time-zero biopsies and allografts with normal or depleted arteriolar SMC TPA.
Experimental Steps: 1. Each experiment included antibody, PBS, and conjugate controls. 2. These were done at room temperature by exposure of cryostat sections to only PBS, isotype-matched irrelevant antibody, or conjugate. 3. The irrelevant rabbit antibody was anti-human IgE (Dakopatts, Glostrup, Denmark), and mouse irrelevant monoclonals were IgGl anti-cytomegalovirus early antigen (Chemicon, Temecula, Calif) and IgG2a anti-Epstein-Barr virus capsid antigen (Chemicon). 4. The dilution for each antibody was two serial dilutions before end point titration. Antibodies were ultracentrifuged at 100 000g at 4C for 1 hour to remove complexes and aggregates, and monthly titrations and ultracentrifugations were done for each antibody. 5. The reactivity of anti-TPA was removed by absorption with recombinant TPA from Genentech (San Francisco, Calif), confirming specificity of the antibody.
Number Of Protocols: 1
Reference1. Goodall, A. H. et al. (1985) An immunoradiometric assay for human factor VIII/von Willebrand factor (VIII:vWF) using a monoclonal antibody that defines a functional epitope. Br. J. Haematol. 59: 565-577.
2. Goodall, A. H. and Meyer, D. (1985) Registry of monoclonal antibodies to factor VIII and von Willebrand factor. International Committee on Thrombosis and Haemostasis. Thromb. Haemost. 54: 878-891.
3. Chand, S. et al. (1986) A two-site, monoclonal antibody-based immunoassay for von Willebrand factor--demonstration that vWF function resides in a conformational epitope. Thromb, Haemost. 55: 318-324.
Gene SymbolVWF
Alias SymbolsVWD, F8VWF
NCBI Gene Id7450
Protein Namevon Willebrand factor
Description of TargetMOUSE ANTI HUMAN VON WILLEBRAND FACTOR
Uniprot IDP04275
Protein Accession #NP_000543.2
Protein Size (# AA)2813
  1. What is the species homology for "VWF Antibody (OASA03379)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "VWF Antibody (OASA03379)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "VWF Antibody (OASA03379)" provided in?

    This item is provided in "Liquid. Phosphate buffered saline".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "VWF Antibody (OASA03379)"?

    This target may also be called "VWD, F8VWF" in publications.

  5. What is the shipping cost for "VWF Antibody (OASA03379)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "VWF Antibody (OASA03379)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "VWF Antibody (OASA03379)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "VWF Antibody (OASA03379)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "VWF"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "VWF"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "VWF"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "VWF"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "VWF"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "VWF"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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