Catalog No: OKEH03464
Size:96T
Price: $525.00
SKU
OKEH03464
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology VWA2 ELISA Kit (Human) (OKEH03464) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VWA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for VWA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VWA2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.8% (n = 20)
Mean Inter-assay CV%: <= 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-VWA2 Microplate96 Wells (12 x 8 Well strips)
VWA2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated VWA2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Human von Willebrand factor A domain-containing protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVWA2
Gene Full Namevon Willebrand factor A domain containing 2
Alias Symbolsa domain-containing protein similar to matrilin and collagen;AMACO;CCSP2;CCSP-2;colon cancer diagnostic marker;colon cancer secreted protein 2;NET42;von Willebrand factor A domain-containing protein 2.
NCBI Gene Id340706
Protein NameQ5GFL6
Description of Targetvon Willebrand factor A domain-containing protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q5GFL6
Protein Accession #NP_001258975.1
Nucleotide Accession #NM_001272046.1
Protein Size (# AA)755
Molecular Weight82 kDa
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