VPX Recombinant Protein (isolate ROD) (OPCA03170)

Catalog No: OPCA03170

vpx Recombinant Protein (Human immunodeficiency virus type 2 subtype A) (OPCA03170) in SDS-PAGE showing the molecular weight of approximately 28.81 kDa.

Product Info

• Predicted Species Reactivity: Human Immunodeficiency Virus Type 2
• Product Format: Liquid or Lyophilized powder
• Host: Human immunodeficiency virus type 2 subtype A
• Reconstitution and Storage: -20°C or -80°C
• Purification: Affinity purified by IMAC
• Concentration: Varies by lot. See vial for concentration.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Peptide Sequence: MTDPRETVPPGNSGEETIGEAFAWLNRTVEAINREAVNHLPRELIFQVWQRSWRYWHDEQGMSESYTKYRYLCIIQKAVYMHVRKGCTCLGRGHGPGGWRPGPPPPPPPGLV
• Protein Sequence: Full Length (1-112aa): MTDPRETVPPGNSGEETIGEAFAWLNRTVEAINREAVNHLPRELIFQVWQRSWRYWHDEQGMSESYTKYRYLCIIQKAVYMHVRKGCTCLGRGHGPGGWRPGPPPPPPPGLV
• Source: E.coli
• Tag: N-terminal 6xHis-SUMO-tagged

Target Info

• Reference: Identification of the nuclear localization signal of human immunodeficiency virus type 2 Vpx.Belshan M., Ratner L.Virology 311:7-15(2003)
• Gene Symbol: VPX
• Alias Symbols: Viral protein X;X ORF protein.
• Protein Name: Protein Vpx
• Description of Target: Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity).
• Uniprot ID: P06939
• Protein Size (# AA): Recombinant
• Molecular Weight: 28.8 kDa