VPS37A ELISA Kit (Human) (OKCD00625)

Catalog No: OKCD00625 (Formerly GWB-KBBRR3)

Size:96WELLS

Human VPS37A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocellular Carcinoma Related Protein 1 (HCRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocellular Carcinoma Related Protein 1 (HCRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocellular Carcinoma Related Protein 1 (HCRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hepatocellular Carcinoma Related Protein 1 (HCRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocellular Carcinoma Related Protein 1 (HCRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocellular Carcinoma Related Protein 1 (HCRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-VPS37A Microplate: 96 Wells (12 x 8 Well Strips)
  • VPS37A Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated VPS37A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocellular Carcinoma Related Protein 1 (HCRP1). No significant cross-reactivity or interference between Hepatocellular Carcinoma Related Protein 1 (HCRP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: VPS37A
• Gene Full Name: VPS37A subunit of ESCRT-I
• Alias Symbols: ESCRT-I complex subunit VPS37A;HCRP1;hepatocellular carcinoma-related protein 1;polyglutamine binding protein 2;PQBP2;SPG53;vacuolar protein sorting 37 homolog A;vacuolar protein sorting-associated protein 37A;VPS37A, ESCRT-I subunit.
• NCBI Gene Id: 137492
• Protein Name: Vacuolar protein sorting-associated protein 37A
• Description of Target: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.
• Uniprot ID: Q8NEZ2
• Protein Accession #: NP_001138624
• Nucleotide Accession #: NM_001145152.1
• Protein Size (# AA): 397