VPS29 ELISA Kit (Human) (OKEH08609)

Catalog No: OKEH08609

Size:96T

Human VPS29 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology VPS29 ELISA Kit (Human) (OKEH08609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VPS29 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VPS29 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VPS29 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.1% (n = 20)
  Mean Inter-assay CV%: <=5.3% (n = 20)
• ELISA Kit Component:
  • VPS29 Microplate: 96 Wells (12 x 8 Well strips)
  • VPS29 Lyophilized Standard: 2
  • 100X Biotinylated VPS29 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (Probable). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27 (PubMed:24856514).
  Subcellular Location: Cytoplasm Membrane Peripheral membrane protein Endosome membrane Peripheral membrane protein Early endosome Late endosome
  Unigene: Hs.600114
  SMR: Q9UBQ0
  String: 9606.ENSP00000380795
  KEGG: hsa:51699
  MIM: 606932
  Pfam: PF12850
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 23pg/mL
• Specificity: Natural and recombinant Human Vacuolar protein sorting-associated protein 29
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: VPS29
• Gene Full Name: VPS29 retromer complex component
• Alias Symbols: DC15;DC7;epididymis secretory sperm binding protein;hVPS29;PEP11;PEP11 homolog;retromer protein;vacuolar protein sorting 29 homolog;vacuolar protein sorting-associated protein 29;vacuolar sorting protein VPS29/PEP11;vesicle protein sorting 29;x 007 protein.
• NCBI Gene Id: 51699
• Protein Name: Q9UBQ0
• Description of Target: Vacuolar protein sorting-associated protein 29
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UBQ0
• Protein Accession #: NP_057310.1
• Nucleotide Accession #: NM_016226.4