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VPS28 Antibody (OAEB02343)

100 ug
$349.00
In Stock
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Predicted Species Reactivity:
Cow, Dog, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
WB, IHC
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Gene Symbol:
VPS28
NCBI Gene Id:
51160
Protein Name:
Vacuolar protein sorting 28 (Yeast), isoform CRA_a EMBL EAW82094.1
Swissprot Id:
Q548N1
Protein Accession #:
NP_057292.1
Replacement Item:
This antibody may replace item sc-166514 from Santa Cruz Biotechnology.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express VPS28.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express VPS28.
Immunogen:
This antibody is expected to recognize isoform 1 (NP_057292.1) only.
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data:
VPS28 Antibody (OAEB02343)
Peptide Sequence:
C-ESAYNAFNRFLHA
Datasheets/Manuals:
Printable datasheet for anti-VPS28 antibody - OAEB02343
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:1000.
Western Blot: Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration, 10ug/ml.
Target Reference:
* Bishop N, Woodman P.; TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.; J Biol Chem. 2001 Apr 13;276(15):11735-42.; Pubmed 11134028

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