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| Datasheets/Manuals | Printable datasheet for VPS11 Antibody (OAEB00467) |
|---|
| Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | IHC |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | VPS11 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100% |
| Peptide Sequence | C-HALQEKDTQTKLE |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. |
| Reference | * Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C.; Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7.; J Biol Chem. 2001 Aug 3;276(31):29393-402. Epub 2001 May 29.; Pubmed 11382755 |
| Gene Symbol | Vps11|VPS11 |
|---|---|
| Gene Full Name | VPS11 core subunit of CORVET and HOPS complexes|VPS11, CORVET/HOPS core subunit |
| Alias Symbols | 1200011A11Rik;END1;HLD12;hVPS11;PEP5;RING finger protein 108;RNF108;vacuolar protein sorting 11;vacuolar protein sorting 11 homolog;vacuolar protein sorting-associated protein 11 homolog;VPS11, CORVET/HOPS core subunit. |
| NCBI Gene Id | 55823|71732 |
| Protein Name | Vacuolar protein sorting 11 (Yeast homolog) variant EMBL BAD96878.1 |
| Uniprot ID | Q53FX3 |
| Protein Accession # | NP_068375.3 |
| Molecular Weight | 108 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "VPS11 Antibody (OAEB00467)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Rat".
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How long will it take to receive "VPS11 Antibody (OAEB00467)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "VPS11 Antibody (OAEB00467)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "VPS11 Antibody (OAEB00467)"?
This target may also be called "1200011A11Rik;END1;HLD12;hVPS11;PEP5;RING finger protein 108;RNF108;vacuolar protein sorting 11;vacuolar protein sorting 11 homolog;vacuolar protein sorting-associated protein 11 homolog;VPS11, CORVET/HOPS core subunit." in publications.
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What is the shipping cost for "VPS11 Antibody (OAEB00467)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "VPS11 Antibody (OAEB00467)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "VPS11 Antibody (OAEB00467)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "108".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "VPS11 Antibody (OAEB00467)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Vps11|VPS11"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Vps11|VPS11"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Vps11|VPS11"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Vps11|VPS11"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Vps11|VPS11"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Vps11|VPS11"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.