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Catalog No: OKCD08193
Size:96WELLS
Price: $825.00
SKU
OKCD08193
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Datasheets/ManualsPrintable datasheet for VNN1 ELISA Kit (Mouse) (OKCD08193)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vanin 1 (VNN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vanin 1 (VNN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vanin 1 (VNN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vanin 1 (VNN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vanin 1 (VNN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vanin 1 (VNN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
VNN1 Microplate96 Wells (12 x 8 Well strips)
VNN1 Lyophilized Standard2
100X VNN1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.1pg/mL
Gene SymbolVnn1
Gene Full Namevanin 1
Alias Symbolspantetheinase;pantetheine hydrolase;V-;V-1;vascular non-inflammatory molecule 1.
NCBI Gene Id22361
Protein NamePantetheinase
Description of TargetAmidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
Uniprot IDQ9Z0K8
Protein Accession #NP_035834.2
Nucleotide Accession #NM_011704.3
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