VKORC1 ELISA Kit (Human) (OKCD08997)

Catalog No: OKCD08997

Size:96WELLS

VKORC1 ELISA Kit (Human) (OKCD08997) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • VKORC1 Microplate: 96 Wells (12 x 8 Well strips)
  • VKORC1 Lyophilized Standard: 2
  • 100X VKORC1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.60ng/mL

Target Info

• Gene Symbol: VKORC1
• Gene Full Name: vitamin K epoxide reductase complex subunit 1
• Alias Symbols: EDTP308;MST134;MST576;phylloquinone epoxide reductase;vitamin K dependent clotting factors deficiency 2;vitamin K epoxide reductase complex subunit 1;vitamin K1 2,3-epoxide reductase subunit 1;vitamin K1 epoxide reductase (warfarin-sensitive);VKCFD2;VKOR.
• NCBI Gene Id: 79001
• Protein Name: Vitamin K epoxide reductase complex subunit 1
• Description of Target: Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Vitamin K is required for the gamma-carboxylation of various proteins, including clotting factors, and is required for normal blood coagulation, but also for normal bone development.
• Uniprot ID: Q9BQB6
• Protein Accession #: NP_001298240.1
• Nucleotide Accession #: NM_001311311.1