Catalog No: OPCA04321
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OPCA04321
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
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Datasheets/Manuals | Printable datasheet for VJBR Recombinant Protein (Brucella abortus ) (OPCA04321) (OPCA04321) |
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Predicted Species Reactivity | Brucella abortus|Brucella melitensis |
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Product Format | Liquid or Lyophilized powder |
Additional Information | Species Specificity Detail: Brucella abortus |
Reconstitution and Storage | -20°C or -80°C |
Formulation | Tris-base, 50% glycerol |
Purity | Greater than 90% as determined by SDS-PAGE. |
Peptide Sequence | MSLDLVHFPNYKKTFFGSSFQSDTLALLTRIRDEIGCRYVTHTYRGRVGDCTKVNSADLTVLMTLPATWVARYSSKNYFAIDPVFQEDAPYYRNDTSAIARDLKEDADICPAVAELLHDAEKHGLGNLFIAVSARNPKGVAGCTVFTFEVEDEDRTQFLARMRPRLLSLAGIIHGTVCGCKDANSVASLLTPREVDCLRWAANGKTDGEIAEILSIARWTVVTYLQNAKIKLNCSNRTSAVATALSLGIIDMPEVQHLV |
Protein Sequence | MSLDLVHFPNYKKTFFGSSFQSDTLALLTRIRDEIGCRYVTHTYRGRVGDCTKVNSADLTVLMTLPATWVARYSSKNYFAIDPVFQEDAPYYRNDTSAIARDLKEDADICPAVAELLHDAEKHGLGNLFIAVSARNPKGVAGCTVFTFEVEDEDRTQFLARMRPRLLSLAGIIHGTVCGCKDANSVASLLTPREVDCLRWAANGKTDGEIAEILSIARWTVVTYLQNAKIKLNCSNRTSAVATALSLGIIDMPEVQHLV |
Storage Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Source | Yeast |
Protein Range | 1-259 aa |
Tag | N-terminal 6xHis-tagged |
Reference | Mutations of the quorum sensing-dependent regulator VjbR lead to drastic surface modifications in Brucella melitensis.Uzureau S., Godefroid M., Deschamps C., Lemaire J., De Bolle X., Letesson J.-J.J. Bacteriol. 189:6035-6047(2007) |
Gene Symbol | vjbR |
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Gene Full Name | HTH-type quorum sensing-dependent transcriptional regulator VjbR |
Alias Symbols | BME_RS15625;BMEII1116;HTH-type quorum sensing-dependent transcriptional regulator VjbR. |
NCBI Gene Id | 29595784 |
Protein Name | HTH-type quorum sensing-dependent transcriptional regulator VjbR |
Description of Target | Transcriptional regulator involved in the global control of Brucella gene expression (PubMed:28334833). Mediates the effects of the quorum sensing autoinducer C12-HSL (N-dodecanoyl-homoserine lactone) on a large and diverse number of genes (By similarity). Binds with high specificity to a large number of sites distributed across the genome. The VjbR-binding motif contains an asymmetric 16-bp consensus sequence, an unusual feature among LuxR-type regulators (PubMed:28334833). May directly modulate expression of many genes, including virulence determinants, components of the respiratory chain, efflux systems, elements predicted to participate in ion transport and adaptation to stress, uncharacterized proteins, as well as transcriptional regulators probably responsible for the observed indirect VjbR-mediated effects (PubMed:28334833). |
Uniprot ID | Q2YJ50 |
Protein Accession # | WP_002966460 |
Nucleotide Accession # | NZ_KN046823 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 30.6 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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