Vitamin B6 ELISA Kit (OKEH02570)

Catalog No: OKEH02570

Size:96T

Vitamin B6 ELISA Kit (OKEH02570) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Vitamin B6 ELISA Kit (OKEH02570) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin B6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B6 and incubated. The Vitamin B6 found in the sample or standards competes with the biotinylated Vitamin B6 for limited binding sites on the immobilized anti-Vitamin B6 antibody. Excess unbound biotinylated Vitamin B6 and sample or standard Vitamin B6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B6 captured in the well and inversely proportional to the amount of Vitamin B6 which was contained in the sample or standard.
• ELISA Kit Range: 3.12-200nmol/L
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • Anti-Vitamin B6 Microplate: 96 Wells (12 x 8 Well strips)
  • Vitamin B6 Lyophilized Standard: 2 x 200 nmol
  • Sample Diluent: 1 x 20 mL
  • 100X Vitamin B6-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: VB6
  Target Name: Vitamin B6
  Pubchem: 1054
  Chemical formula: C8H11NO3
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 1.26nmol/L
• Specificity: Natural and recombinant General Vitamin B6
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: VB6, Vitamin B6, VB6
• Protein Name: Vitamin B6
• Description of Target: Vitamin B6 is a water-soluble vitamin and is part of the vitamin B complex group. Several forms of the vitamin are known, but pyridoxal phosphate (PLP) is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. PLP also is necessary for the enzymatic reaction governing the release of glucose from glycogen.