Vitamin B1 ELISA Kit (OKEH02610)

Catalog No: OKEH02610

Size:96T

Vitamin B1 ELISA Kit (OKEH02610) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Vitamin B1 ELISA Kit (OKEH02610) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin B1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B1 and incubated. The Vitamin B1 found in the sample or standards competes with the biotinylated Vitamin B1 for limited binding sites on the immobilized anti-Vitamin B1 antibody. Excess unbound biotinylated Vitamin B1 and sample or standard Vitamin B1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B1 captured in the well and inversely proportional to the amount of Vitamin B1 which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 110%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.8% (n = 20)
  Mean Inter-assay CV%: < 8.4% (n = 20)
• ELISA Kit Component:
  • Anti-Vitamin B1 Microplate: 96 Wells (12 x 8 Well strips)
  • Vitamin B1 Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Vitamin B1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: VB1
  Target Name: Vitamin B1
  Pubchem: 6042
  Chemical formula: C12H17N4OS+
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.36 ng/mL
• Specificity: Natural and recombinant General Vitamin B1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: VB1, Vitamin B1, VB1, Thiamine, thiamin
• Protein Name: Vitamin B1
• Description of Target: Thiamine is a heat-labile and water-soluble essential vitamin, belonging to the vitamin B family, with antioxidant, erythropoietic, mood modulating, and glucose-regulating activities. Thiamine reacts with adenosine triphosphate (ATP) to form an active coenzyme, thiamine pyrophosphate. Thiamine pyrophosphate is necessary for the actions of pyruvate dehydrogenase and alpha-ketoglutarate in carbohydrate metabolism and for the actions of transketolase, an enzyme that plays an important role in the pentose phosphate pathway. Thiamine plays a key role in intracellular glucose metabolism and may inhibit the action of glucose and insulin on arterial smooth muscle cell proliferation. Thiamine may also protect against lead toxicity by inhibiting lead-induced lipid peroxidation.