VISFATIN Recombinant Protein (OPSA11048)

Catalog No: OPSA11048 (Formerly GWB-A439E1)

Size:25UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized. 10mM HCl
• Application: Functional assay
• Additional Information: Activity: The ED ₅₀ was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED ₅₀ for this effect is 15.0-20.0 ng/ml.
  Approx Protein Conc: Total protein concentration 0.1mg/ml after reconstitution.
  Buffer solution: 10mM Tris
  25mM NaCl
  Endotoxin Level: < 1EU/ug
  Label Protein Molar Ratio: Purified recombinant visfatin expressed in E.coli.
  Preservative: None present
• Reconstitution and Storage: -20°C
• Predicted Homology Based on Immunogen Sequence: Human
• Purification: Purified recombinant visfatin expressed in E.coli
• Concentration: 0.1 mg/ml
• Purity: >98% by SDS PAGE and HPLC analysis.
• Specificity: Visfatin is a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide.
  Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes.
  

Target Info

• Gene Symbol: NAMPT
• Alias Symbols: VF, PBEF, PBEF1, VISFATIN, 1110035O14Rik
• NCBI Gene Id: 10135
• Protein Name: Nicotinamide phosphoribosyltransferase
• Description of Target: RECOMBINANT HUMAN VISFATIN
  
• Uniprot ID: P43490
• Protein Accession #: NP_005737.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 52.5kD (465 amino acid residues).