VIPR1 ELISA kit (Human) (OKCD08190)

Catalog No: OKCD08190

Size:96WELLS

VIPR1 ELISA Kit (Human) (OKCD08190) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasoactive Intestinal Peptide Receptor 1 (VIPR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasoactive Intestinal Peptide Receptor 1 (VIPR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasoactive Intestinal Peptide Receptor 1 (VIPR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vasoactive Intestinal Peptide Receptor 1 (VIPR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide Receptor 1 (VIPR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide Receptor 1 (VIPR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • VIPR1 Microplate: 96 Wells (12 x 8 Well strips)
  • VIPR1 Lyophilized Standard: 2
  • 100X VIPR1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: VIPR1
• Gene Full Name: vasoactive intestinal peptide receptor 1
• Alias Symbols: II, HVR1, RDC1, V1RG, VIPR, VIRG, VAPC1, VPAC1, VPAC1R, VIP-R-1, VPCAP1R, PACAP-R2, PACAP-R-2
• NCBI Gene Id: 7433
• Protein Name: Vasoactive intestinal polypeptide receptor 1
• Description of Target: This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P32241
• Protein Accession #: NP_001238811.1
• Nucleotide Accession #: NM_001251882.1