VIP ELISA kit (Bovine) (OKCD02980)

Catalog No: OKCD02980

Size:96WELLS

VIP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-97%	96-105%	79-95%	78-94%
  EDTA Plasma (n=5)	78-97%	90-101%	81-94%	79-94%
  Heparin Plasma (n=5)	78-94%	78-95%	79-92%	96-103%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vasoactive Intestinal Peptide (VIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vasoactive Intestinal Peptide (VIP) and unlabeled Vasoactive Intestinal Peptide (VIP) (Standards or samples) with the pre-coated antibody specific to Vasoactive Intestinal Peptide (VIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vasoactive Intestinal Peptide (VIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vasoactive Intestinal Peptide (VIP) in the sample.
• ELISA Kit Range: 6.17-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-99	90
  EDTA Plasma (n=5)	86-95	91
  Heparin Plasma (n=5)	82-104	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-VIP Microplate: 96 Wells (12 x 8 Well strips)
  • VIP Lyophilized Standard: 2 x 500 pg
  • 100X VIP Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 2.63 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP). No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: VIP
• Gene Full Name: vasoactive intestinal peptide
• Alias Symbols: VIP peptides.
• NCBI Gene Id: 280956
• Protein Name: VIP peptides
• Description of Target: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
• Uniprot ID: P81401
• Protein Accession #: NP_776395.1
• Nucleotide Accession #: NM_173970.3
• Molecular Weight: 170 kDa