- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Vinculin Antibody - N-terminal (OAPB02067) (OAPB02067) |
---|
Predicted Species Reactivity | Chicken|Human|Mouse|Porcine|Rabbit|Rat |
---|---|
Product Format | Liquid. Vinculin Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Monoclonal |
Isotype | IgG |
Host | Mouse |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Western blot |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin. The immunogen is located within amino acids 50 - 100 of Vinculin. |
Purification | Protein A Purified |
Concentration | 1 mg/ml |
Gene Symbol | VCL |
---|---|
Gene Full Name | vinculin |
Alias Symbols | CMD1W;CMH15;epididymis luminal protein 114;epididymis secretory sperm binding protein;HEL114;metavinculin;meta-vinculin;MV;MVCL;vinculin;vinculin isoform meta-VCL. |
NCBI Gene Id | 7414 |
Protein Name | vinculin isoform VCL [Homo sapiens] |
Description of Target | Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5). |
Uniprot ID | P18206 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_003364 |
Nucleotide Accession # | NM_003373.3 |
Molecular Weight | Predicted: 117 kDa Observed: 120 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Vinculin Antibody - N-terminal (OAPB02067)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Chicken|Human|Mouse|Porcine|Rabbit|Rat".
-
How long will it take to receive "Vinculin Antibody - N-terminal (OAPB02067)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
-
What buffer format is "Vinculin Antibody - N-terminal (OAPB02067)" provided in?
This item is provided in "Liquid. Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Vinculin Antibody - N-terminal (OAPB02067)"?
This target may also be called "CMD1W;CMH15;epididymis luminal protein 114;epididymis secretory sperm binding protein;HEL114;metavinculin;meta-vinculin;MV;MVCL;vinculin;vinculin isoform meta-VCL." in publications.
-
What is the shipping cost for "Vinculin Antibody - N-terminal (OAPB02067)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Vinculin Antibody - N-terminal (OAPB02067)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Vinculin Antibody - N-terminal (OAPB02067)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 117 kDa Observed: 120 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Vinculin Antibody - N-terminal (OAPB02067)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "VCL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "VCL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "VCL"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "VCL"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "VCL"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "VCL"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.