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Datasheets/Manuals | Printable datasheet for anti-Vimentin antibody - OAPA00121 |
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Predicted Species Reactivity | Human |
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Clonality | Monoclonal |
Clone | hVimentin. |
Isotype | Mouse IgG1. |
Host | Mouse |
Application | WB, IP, IHC |
Additional Information | Titer: For intra-cellular staining, use 5-10 ul/106 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent. |
:: | Product Type: Mouse Anti Human Monoclonal Antibody. Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4C. |
:: | Product Introduction: Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. |
Reconstitution and Storage | In lyophilized form, for long periods, store at 4C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20C. Shipping Conditions: Antibody is shipped lyophilized at ambient temperature. |
Immunogen | The immunogen for anti-Vimentin antibody: purified Human Vimentin. |
Predicted Homology Based on Immunogen Sequence | Vimentin antibody will recognize most mammalian Vimentin except mouse and rat. |
Purity | Ion exchange column. |
Gene Symbol | Vimentin |
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Alias Symbols | Vimentin Antibody, Vimentin, Mouse Anti Human Antibody |
NCBI Gene Id | 7431 |
Protein Name | Vimentin |
Description of Target | Mouse Anti Human Vimentin |
Uniprot ID | P08670 |
Protein Accession # | NP_003371.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Vimentin Antibody (OAPA00121)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Vimentin Antibody (OAPA00121)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Vimentin Antibody (OAPA00121)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Vimentin Antibody (OAPA00121)"?
This target may also be called "Vimentin Antibody, Vimentin, Mouse Anti Human Antibody" in publications.
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What is the shipping cost for "Vimentin Antibody (OAPA00121)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Vimentin Antibody (OAPA00121)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Vimentin Antibody (OAPA00121)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Vimentin Antibody (OAPA00121)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "VIMENTIN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "VIMENTIN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "VIMENTIN"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "VIMENTIN"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "VIMENTIN"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "VIMENTIN"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.