VHL ELISA Kit (Rat) (OKEH07205)

Catalog No: OKEH07205

Size:96T

Rat VHL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Vhl ELISA Kit (Rat) (OKEH07205) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vhl has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vhl is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vhl captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 100%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <4.7%
  Inter-Assay CV <7.2%
• ELISA Kit Component:
  • Vhl Microplate: 96 Wells (12 x 8 Well strips)
  • Vhl Lyophilized Standard: 2 x 10 ng
  • 100X Vhl HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.087 ng/mL
• Specificity: Natural and recombinant Rat Von Hippel-Lindau disease tumor suppressor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Vhl
• Gene Full Name: von Hippel-Lindau tumor suppressor
• Alias Symbols: pVHL;Vhlh;von Hippel-Lindau disease tumor suppressor;von Hippel-Lindau protein;von Hippel-Lindau syndrome homolog;von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase.
• NCBI Gene Id: 24874
• Protein Name: Q64259
• Description of Target: von Hippel-Lindau disease tumor suppressor
• Uniprot ID: https://www.uniprot.org/uniprot/Q64259
• Protein Accession #: NP_434688
• Nucleotide Accession #: NM_052801