VGLUT2 Antibody : HRP (OASE00243-HRP)

Catalog No: OASE00243-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S29-29
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: IHC, WB
• Additional Information: Background Info: Detects ~60kDa.
  Scientific Background: The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi)
  cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic alpha and beta cells express BNPI and
  VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
  
  Certificate of Analysis: 1ug/mL was sufficient for detection of VGLut2 in 20ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2.
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250.
  3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451.
  4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803.
  5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391.
  6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.
  

Target Info

• Gene Symbol: Slc17a6
• Gene Full Name: solute carrier family 17 (vesicular glutamate transporter), member 6
• Alias Symbols: Dnpi, Vglut2
• NCBI Gene Id: 84487
• Uniprot ID: Q9JI12
• Protein Accession #: NP_445879.1