VGLUT1 Antibody (OASE00242)

Catalog No: OASE00242

Size:100ug

Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82. Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) MMP9 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS, pH 7.4 with 50% glycerol and 0.09% sodium azide.
• Clonality: Monoclonal
• Clone: S28-9
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: IHC, WB
• Additional Information: Certificate of Analysis: 1ug/mL was sufficient for detection of VGLut1 in 20ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20°C for 1 year. Avoid repeated freeze/thaw cycles.
• Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~52kDa. No cross-reactivity against VGlut2.
• Application Data: WB 1:1000
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Description: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

Target Info

• Reference: 1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163.
  2.Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.
• Gene Symbol: VGLUT1
• Gene Full Name: solute carrier family 17 (vesicular glutamate transporter), member 7
• Alias Symbols: BNPI, SLC17A1, SLC17A1/VGLUT1, Solute Carrier family 17 member 7, Vesicular glutamate transporter 1, VGLUT 1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter
• NCBI Gene Id: 116638
• Description of Target: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
• Uniprot ID: Q62634
• Protein Accession #: NP_446311.1