VGF ELISA Kit (Rat) (OKCD02976)

Catalog No: OKCD02976

Size:96WELLS

Rat VGF ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-105%	79-101%	78-89%	80-97%
  EDTA Plasma (n=5)	97-104%	84-104%	96-103%	81-89%
  Heparin Plasma (n=5)	92-101%	81-94%	96-103%	90-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF Nerve Growth Factor Inducible (VGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VGF Nerve Growth Factor Inducible (VGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF Nerve Growth Factor Inducible (VGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VGF Nerve Growth Factor Inducible (VGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-95	92
  EDTA Plasma (n=5)	88-101	93
  Heparin Plasma(n=5)	94-105	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-VGF Microplate: 96 Wells (12 x 8 Well strips)
  • VGF Lyophilized Standard: 2
  • 100X Biotinylated VGF Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
• Sensitivity: 18.34 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of VGF Nerve Growth Factor Inducible (VGF). No significant cross-reactivity or interference between VGF Nerve Growth Factor Inducible (VGF) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Vgf
• Gene Full Name: VGF nerve growth factor inducible
• Alias Symbols: neurosecretory protein VGF;neurosecretory protein VGF-like;VGF8a protein.
• NCBI Gene Id: 29461
• Protein Name: Neurosecretory protein VGF
• Description of Target: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
• Uniprot ID: P20156
• Protein Accession #: NP_112259.1
• Nucleotide Accession #: NM_030997.1
• Protein Size (# AA): 617