Catalog No: OKCD07180
Size:96WELLS
Price: $750.00
SKU
OKCD07180
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for VGF ELISA Kit (Human) (OKCD07180) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VGF Nerve Growth Factor Inducible (VGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VGF Nerve Growth Factor Inducible (VGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VGF Nerve Growth Factor Inducible (VGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VGF Nerve Growth Factor Inducible (VGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 78-5,000pg/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VGF Nerve Growth Factor Inducible (VGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, cerebrospinal fluid and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 28pg/mL |
Gene Symbol | VGF |
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Gene Full Name | VGF nerve growth factor inducible |
Alias Symbols | neuro-endocrine specific protein VGF;neurosecretory protein VGF;SCG7;SgVII. |
NCBI Gene Id | 7425 |
Protein Name | Neurosecretory protein VGF |
Description of Target | May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. |
Uniprot ID | O15240 |
Protein Accession # | NP_003369.2 |
Nucleotide Accession # | NM_003378.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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