Versican Antibody (OASE00287)

Catalog No: OASE00287

Size:100ug

Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:250.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GFAP Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S351-23
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, ICC, IF
• Additional Information: Background Info: Detects >350kDa
  Scientific Background: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth
  and differentiation. Important as a structural molecule,
  versican creates loose and hydrated matrices during key
  events in development and disease. The protein contains
  hyaluronic acid and glycosminoglycan-binding domains,
  epidermal growth factor-like repeats, a Lectinlike
  sequence and a complement regulatory protein-like
  domain. Splice variants differ greatly in length and
  degree of modification by glycoaminoglycan chains.
  Accumulation around smooth muscle cells in lesions of
  athero-sclerosis suggests a role for versican in
  atherogenesis. Versican, differentially expressed in
  human melanoma, plays a role in tumor development
  and may be a reliable marker for clinical diagnosis. The
  organization of HA- and versican-rich pericellular
  matrices may faciliatate migration and mitosis by
  diminishing cell surface adhesivity and affecting cell
  shape through steric exclusion and the viscous properties of HA proteoglycan gels.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Versican in 20ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects >350kDa.
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Reference: 1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998.
  2. Evanko S.P., Angello J.C. and Wight T.N. (1999)
  Arterioscler. Thromb. Vasc. Biol. 4:1004-1013.
  3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc.
  Biol. 7: 1630-1639.
  4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623.
  5. Touab M., Villena J., Barranco C., Arumi-Uria M. and
  Bassols A. (2002) Am. J. Pathol. 2: 549-557.
• Description: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.

Target Info

• Gene Symbol: Versican
• Gene Full Name: versican
• Alias Symbols: Chondroitin sulfate proteoglycan 2, CSPG2, ERVR, GHAP, PG-M, VCAN, Chondroitin sulfate proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate binding protein, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, Large fibroblast proteoglycan, PGM, V1 Neo, Versican core protein, Versican proteoglycan, Versican V0, WGN 1, WGN, WGN1
• NCBI Gene Id: 13003
• Uniprot ID: Q62059
• Nucleotide Accession #: AAH96495