Versican Antibody : FITC (OASE00287-FITC)

Catalog No: OASE00287-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S351-23
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, ICC
• Additional Information: Background Info: Detects >350kDa
  Scientific Background: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth
  and differentiation. Important as a structural molecule,
  versican creates loose and hydrated matrices during key
  events in development and disease. The protein contains
  hyaluronic acid and glycosminoglycan-binding domains,
  epidermal growth factor-like repeats, a Lectinlike
  sequence and a complement regulatory protein-like
  domain. Splice variants differ greatly in length and
  degree of modification by glycoaminoglycan chains.
  Accumulation around smooth muscle cells in lesions of
  athero-sclerosis suggests a role for versican in
  atherogenesis. Versican, differentially expressed in
  human melanoma, plays a role in tumor development
  and may be a reliable marker for clinical diagnosis. The
  organization of HA- and versican-rich pericellular
  matrices may faciliatate migration and mitosis by
  diminishing cell surface adhesivity and affecting cell
  shape through steric exclusion and the viscous properties of HA proteoglycan gels.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Versican in 20ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein, accession number Q62059.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Reference: 1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998.
  2. Evanko S.P., Angello J.C. and Wight T.N. (1999)
  Arterioscler. Thromb. Vasc. Biol. 4:1004-1013.
  3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc.
  Biol. 7: 1630-1639.
  4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623.
  5. Touab M., Villena J., Barranco C., Arumi-Uria M. and
  Bassols A. (2002) Am. J. Pathol. 2: 549-557.

Target Info

• Gene Symbol: Vcan
• Gene Full Name: versican
• Alias Symbols: Csp, DPE, NG2, PG-, hdf, PG-M, Cspg2, DPEAAE, PG-M(V0), PG-M(V1), 9430051N09, 5430420N07Rik
• NCBI Gene Id: 13003
• Uniprot ID: Q62059
• Protein Accession #: AAH96495