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Catalog No: OKDD00631 (Formerly GWB-KBBXF3)
Size:96 Wells
Price: $450.00
SKU
OKDD00631
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Datasheets/ManualsPrintable datasheet for VEGFR2 ELISA Kit (Mouse) (OKDD00631)
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology VEGFR2 ELISA Kit (Mouse) (OKDD00631) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for VEGFR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VEGFR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample VEGFR2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-VEGFR2 Microplate96 Wells (12 x 8 Well Strips)
VEGFR2 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X VEGFR2 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates
Sensitivity<0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of VEGFR2. No significant cross-reactivity or interference between VEGFR2 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolKdr
Gene Full Namekinase insert domain protein receptor
Alias Symbols6130401C07;fetal liver kinase 1;Flk;Flk-;Flk1;Flk-1;kinase NYK;Krd-1;Ly73;orv;protein-tyrosine kinase receptor flk-1;soluble vascular endothelial growth factor receptor 2;sVEGFR-2;vascular endothelial growth factor receptor 2;vascular endothelial growth factor receptor-3;VEGF;VEGF receptor-2;VEGFR;VEGFR2;VEGFR-2.
NCBI Gene Id16542
Protein NameKinase insert domain protein receptor
Uniprot IDQ8VCD0
Protein Accession #NP_034742.2
Nucleotide Accession #NM_010612.2
Protein Size (# AA)1345
Molecular Weight150 kDa
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