Catalog No: OKCD04022
Size:96WELLS
Price: $793.00
SKU
OKCD04022
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VEGFA Chemi-Luminescent ELISA Kit (Mouse) (OKCD04022)

Datasheets/ManualsPrintable datasheet for VEGFA Chemi-Luminescent ELISA Kit (Mouse) (OKCD04022)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%81-92%93-101%78-97%
EDTA Plasma (n=5)97-105%79-96%90-97%85-101%
Heparin Plasma (n=5)97-105%95-102%95-103%78-104%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor A (VEGFA) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-9993
EDTA Plasma (n=5)94-10197
Heparin Plasma (n=5)85-9490
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Vegfa Microplate96 Wells (12 x 8 Well strips)
Vegfa Lyophilized Standard2
100X Vegfa Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity1.37 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVegfa
Gene Full Namevascular endothelial growth factor A
Alias SymbolsV;vascular endothelial growth factor A;vascular permeability factor;Veg;Vegf;VEGF12;VEGF16;VEGF18;Vpf.
NCBI Gene Id22339
Protein NameVascular endothelial growth factor A
Description of TargetGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (PubMed:26503042).
Uniprot IDQ00731
Protein Accession #NP_001020421
Nucleotide Accession #NM_001025250
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