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Vegfa Antibody (OASA08493)

0.1 mg
$609.00
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Predicted Species Reactivity:
Mouse
Product Format:
Purified IgG - lyophilised
Clonality:
Polyclonal
Isotype:
Polyclonal IgG
Host:
Rabbit
Application:
ELISA, WB, IHC-P, IF
Additional Information:
Note: Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
For long term
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml after reconstitution.
Buffer Solutions: Phosphate buffered saline
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1 ml distilled water
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Gene Symbol:
Vegfa
NCBI Gene Id:
22339
Protein Name:
Vascular endothelial growth factor A
Swissprot Id:
Q00731
Protein Accession #:
NP_001020421.2
Alias Symbols:
Vpf, Vegf, Vegf120, Vegf164, Vegf188, Vegfa
Replacement Item:
This antibody may replace item sc-152 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI MOUSE VEGF
Protein Size (# AA):
392
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Vegfa.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Vegfa.
Immunogen:
The immunogen for anti-Vegfa antibody: recombinant mouse VEGF
Predicted Homology Based on Immunogen Sequence:
Mouse
Datasheets/Manuals:
Printable datasheet for anti-Vegfa antibody - OASA08493
Specificity:
OASA08493 is specific for mouse Vascular Endothelial Growth Factor (VEGF)
VEGF is a homodimeric protein expressed by various vascularized tissues.
VEGF is reported to stimulate endothelial cell growth and angiogenesis.
Application Info:
Western Blotting : 0.1ug/ml - 0.2ug/ml
Immunofluorescence: 2.5ug/ml
ELISA : 0.5ug/ml
This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA with OASA08494 as a detection reagent and OPSA11123 as the standard.
Immunohistology - Paraffin: 2.5ug/ml
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Histology Positive Control Tissue : Kidney

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