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Catalog No: OKRC01327

Size:96 Wells

Price: $537.00

SKU

OKRC01327

Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks

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VEGF ELISA Kit (Mouse) (OKRC01327)

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Technical Documents

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info
Tested Species Reactivity	Mouse
Predicted Species Reactivity	Mouse
Application	ELISA
ELISA Kit Detection Method	Colorimetric, OD450 nm
ELISA Kit Range	2 pg/ml - 1000 pg/ml
ELISA Kit Component	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Additional Information	Notes: Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
::	Other materials required: Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Reconstitution and Storage	The entire kit may be stored at -20C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4C for up to 6 months. For extended storage, it is recommended to store at -80C.
Sample Type	Cell Culture Supernatants, Plasma, Serum
Sensitivity	2 pg/ml
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Assay Info	Assay Methodology: Quantitative Sandwich ELISA
Dilution	3 fold

Gene Symbol	VEGF
Alias Symbols	V, Veg, Vpf, Vegf, VEGF12, VEGF16, VEGF18
NCBI Gene Id	22339
Uniprot ID	Q00731

Protocols & Data

Protocol:

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