Catalog No: OKEH00466
Size:96T
Price: $525.00
SKU
OKEH00466
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VDR ELISA Kit (Human) : 96 Wells (OKEH00466)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology VDR ELISA Kit (Human) (OKEH000466) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog VDR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated VDR and incubated. The VDR found in the sample or standards competes with the biotinylated VDR for limited binding sites on the immobilized anti-dog VDR antibody. Excess unbound biotinylated VDR and sample or standard VDR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaVDRion is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color produVDR that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated VDR captured in the well and inversely proportional to the amount of VDR which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 10.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-VDR Microplate96 Wells (12 x 8 Well strips)
VDR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X VDR-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.16 ng/mL
SpecificityNatural and recombinant Human Vitamin D3 receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVDR
Gene Full Namevitamin D receptor
Alias Symbols1,25-dihydroxyvitamin D3 receptor;NR1I1;nuclear receptor subfamily 1 group I member 1;PPP1R163;protein phosphatase 1, regulatory subunit 163;vitamin D (1,25- dihydroxyvitamin D3) receptor;vitamin D nuclear receptor variant 1;vitamin D3 receptor;vitamin D3 receptor isoform VDRA.
NCBI Gene Id7421
Protein NameP11473
Description of TargetVitamin D3 receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P11473
Protein Accession #NP_000367.1
Nucleotide Accession #NM_000376.2
Protein Size (# AA)427
Molecular Weight48 kDa
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