VDAC2 Antibody - C-terminal region (OAGA02386)

Catalog No: OAGA02386

Size:100 ul

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
OAGA02386 diluted at 1:1000

VDAC2 antibody [C2C3], C-term detects VDAC2 protein by western blot analysis.
A. 30 ug A549 whole cell lysate/extract
12 % SDS-PAGE
VDAC2 antibody [C2C3], C-term (OAGA02386) dilution: 1:1000

VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria on normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
VDAC2 antibody [C2C3], C-term (OAGA02386) dilution: 1:250.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using VDAC2 (OAGA02386) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.

VDAC2 antibody [C2C3], C-term immunoprecipitates VDAC2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of VDAC2 protein by 4 ug VDAC2 antibody [C2C3], C-term (OAGA02386)
10 % SDS-PAGE
The immunoprecipitated VDAC2 protein was detected by VDAC2 antibody [C2C3], C-term (OAGA02386) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1X PBS, 20% Glycerol (pH7). ProClin300 was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-FFPE, ICC, IP, WB
  Positive Controls: A549, H1299, HCT116, mouse liver
  Cellular Location: Mitochondrion outer membrane
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VDAC2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Rat (92%), Pig (92%), Rabbit (92%), Rhesus Monkey (92%), Bovine (92%)
• Concentration: 0.89 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  IP: 1:100~500
  WB: 1:500~3000
  ELISA: Assay-dependent dilution

Target Info

• Gene Symbol: VDAC2
• Gene Full Name: voltage-dependent anion channel 2
• Alias Symbols: POR
• NCBI Gene Id: 7417
• Description of Target: This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants.
• Uniprot ID: P45880
• Molecular Weight: 32 kDa