VCP Antibody (OAAB17778)

Catalog No: OAAB17778

Size:400ul

Western blot analysis of lysates from MCF-7, U251, mouse NIH/3T3, rat C6 cell line and mouse liver tissue lysate (from left to right) using VCP Antibody (Cat. # OAAB17778).
OAAB17778 was diluted at 1:1000 at each lane.
A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody.
Lysates at 35ug per lane.
Dilution: 1:2000

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This VCP antibody is generated from a mouse immunized with a recombinant protein from human VCP.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Application Info: IF: 0.059027777777778
  WB: 1:2000
• Reference: Lamerdin J.E., et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
  Hu R.-M., et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
  Ota T., et al.Nat. Genet. 36:40-45(2004).
  Humphray S.J., et al.Nature 429:369-374(2004).
  Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Target Info

• Gene Symbol: VCP
• Alias Symbols: p97, TERA, CDC48, FTDALS6
• NCBI Gene Id: 7415
• Description of Target: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168- dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage.
• Uniprot ID: P55072, Q01853
• Molecular Weight: 89 kDa