VCAN ELISA Kit (Mouse) (OKCD02984)

Catalog No: OKCD02984

Size:96WELLS

Mouse VCAN ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-97%	86-102%	80-103%	95-102%
  EDTA Plasma (n=5)	98-105%	95-102%	79-105%	98-105%
  Heparin Plasma (n=5)	81-91%	87-98%	88-101%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Versican (VCAN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Versican (VCAN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Versican (VCAN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Versican (VCAN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-101	84
  EDTA Plasma (n=5)	86-102	92
  Heparin Plasma(n=5)	79-98	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VCAN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VCAN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-VCAN Microplate: 96 Wells (12 x 8 Well strips)
  • VCAN Lyophilized Standard: 2
  • 100X Biotinylated VCAN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.065 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Versican (VS). No significant cross-reactivity or interference between Versican (VS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: VCAN
• Gene Full Name: versican
• Alias Symbols: Chondroitin sulfate proteoglycan core protein 2;Large fibroblast proteoglycan;PG-M.
• Protein Name: Versican core protein
• Description of Target: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
• Uniprot ID: Q62059
• Protein Accession #: NP_001074718.1
• Nucleotide Accession #: NM_001081249.1
• Protein Size (# AA): 3357