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Catalog No: OKCD04027
Size:96WELLS
Price: $800.00
SKU
OKCD04027
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VCAN Chemi-Luminescent ELISA Kit (Human) (OKCD04027)

Datasheets/ManualsPrintable datasheet for VCAN Chemi-Luminescent ELISA Kit (Human) (OKCD04027)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-104%83-97%85-99%82-102%
EDTA Plasma (n=5)80-98%86-101%80-91%99-105%
Heparin Plasma (n=5)95-104%93-101%80-92%78-104%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Versican (VCAN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Versican (VCAN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Versican (VCAN) level in the sample or standard.
ELISA Kit Range6.86-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)94-10197
EDTA Plasma (n=5)94-105101
Heparin Plasma (n=5)85-10495
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VCAN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VCAN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
VCAN Microplate96 Wells (12 x 8 Well strips)
VCAN Lyophilized Standard2
100X VCAN Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.312 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVCAN
Gene Full Nameversican
Alias Symbolschondroitin sulfate proteoglycan core protein 2;CSPG2;ERVR;GHAP;glial hyaluronate-binding protein;large fibroblast proteoglycan;PG-M;versican core protein;versican proteoglycan;WGN;WGN1.
NCBI Gene Id1462
Protein NameVersican core protein
Description of TargetMay play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Uniprot IDP13611
Protein Accession #NP_001119808
Nucleotide Accession #NM_001126336
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