Size:96 Wells
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VCAM1 ELISA Kit (Rat) (OKEH02979)

Catalog#: OKEH02979
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology VCAM1 ELISA Kit (Rat) (OKEH02979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vcam1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Vcam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vcam1 captured in well.
Kit Range 0.312 - 20ng/mL
Kit Component
Anti-Vcam1 Microplate96 Wells (12 x 8 Well strips)
Vcam1 Lyophilized Standard2
100X Biotinylated Vcam1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 0.177 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Rat Vascular cell adhesion protein 1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol VCAM1
Official Gene Full Name vascular cell adhesion molecule 1
Alias Symbols MGC108734, Vascular cell adhesion protein 1, Vcam-1, VCAM-1, V-CAM 1, VCAM1B
NCBI Gene Id 25361
Protein Name Vascular cell adhesion protein 1
Description of Target Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Swissprot Id P29534
Protein Accession # NP_037021.1
Nucleotide Accession # NM_012889.1
Protein Size (# AA) 739
Molecular Weight 81 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Vcam1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Vcam1.
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