Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH04060
Size:96T
Price: $425.00
SKU
OKEH04060
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Vcam1 ELISA Kit (Mouse) (OKEH04060) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vcam1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vcam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vcam1 captured in the well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Vcam1 Microplate96 Wells (12 x 8 Well Strips)
Vcam1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Vcam1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.396 ng/mL
SpecificityNatural and recombinant Mouse Vascular cell adhesion protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVCAM1
Gene Full Namevascular cell adhesion molecule 1
Alias SymbolsVcam, CD106, Vcam-1
NCBI Gene Id22329
Protein NameP29533
Description of TargetVascular cell adhesion protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P29533
Protein Accession #NP_035823.3
Nucleotide Accession #NM_011693.3
Protein Size (# AA)739
Molecular Weight81 kDa
Write Your Own Review
You're reviewing:VCAM1 ELISA Kit (Mouse) (OKEH04060)
Your Rating
We found other products you might like!