Catalog No: OKCD00745 (Formerly GWB-KBBUH7)
Size:96WELLS
Price: $650.00
SKU
OKCD00745
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Datasheets/ManualsPrintable datasheet for VCAM-1 ELISA Kit (Rabbit) (OKCD00745)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-96%78-91%95-103%88-104%
EDTA plasma(n=5)94-105%90-98%93-101%91-98%
heparin plasma(n=5)82-101%86-101%91-103%97-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9692
EDTA plasma(n=5)93-10296
heparin plasma(n=5)89-9693
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-VCAM-1 Microplate96 Wells (12 x 8 Well Strips)
VCAM-1 Lyophilized Standard2 x 2 ng
100X Biotinylated VCAM-1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 14.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1). No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolVCAM1
Gene Full Namevascular cell adhesion molecule 1
Alias Symbolsadhesion molecule VCAM-1;vascular cell adhesion protein 1;VCAM-1.
NCBI Gene Id100008901
Protein NameAdhesion molecule VCAM-1
Description of TargetAdhesion molecule VCAM-1
Uniprot IDQ865F2
Protein Accession #NP_001075621
Nucleotide Accession #NM_001082152.1
Protein Size (# AA)739
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