VBP1 Antibody (OAGA01096)

Catalog No: OAGA01096

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
OAGA01096 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
OAGA01096 diluted at 1:1000

VBP1 antibody detects VBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: VBP1 protein stained by VBP1 antibody (OAGA01096) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq]
  Positive Controls: A549, mouse brain
  Cellular Localization: Cytoplasm, Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 173 of VBP1
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: VBP1
• Gene Full Name: Prefoldin subunit 3
• Alias Symbols: PFD3, PFDN3, VBP-1, HIBBJ46
• NCBI Gene Id: 7411
• Protein Name: Prefoldin subunit 3
• Uniprot ID: P61758
• Protein Accession #: NP_003363.1
• Molecular Weight: 23 kDa